| 20-Aug-49 | Watford | Home | League | 0-0 | | 16522 | |
| 24-Aug-49 | Crystal Palace | Away | League | 1-1 | Neary | 17664 | |
| 27-Aug-49 | Reading | Away | League | 1-5 | Neary | 16717 | |
| 1-Sep-49 | Crystal Palace | Home | League | 2-2 | Neary (pen), Pullen | 14868 | |
| 3-Sep-49 | Torquay United | Home | League | 2-1 | Pullen (2) | 14613 | |
| 6-Sep-49 | Southend United | Away | League | 0-2 | | 12119 | |
| 10-Sep-49 | Exeter City | Home | League | 4-1 | Pullen (2), Smith, Sutherland | 13552 | |
| 17-Sep-49 | Ipswich Town | Away | League | 4-4 | Sutherland (3), Rooney | 13074 | |
| 24-Sep-49 | Port Vale | Home | League | 1-0 | Rooney | 15698 | |
| 1-Oct-49 | Notts County | Away | League | 1-7 | Sutherland | 36436 | |
| 8-Oct-49 | Aldershot | Away | League | 0-2 | | 7223 | |
| 15-Oct-49 | Nottingham Forest | Home | League | 1-1 | Neary | 15767 | |
| 22-Oct-49 | Northampton Town | Away | League | 0-3 | | 11950 | |
| 29-Oct-49 | Norwich City | Home | League | 1-2 | Sherratt | 13210 | |
| 5-Nov-49 | Newport County | Away | League | 2-3 | Sherratt (2) | 11792 | |
| 12-Nov-49 | Bristol City | Home | League | 1-0 | Wood | 9289 | |
| 19-Nov-49 | Brighton & Hove Albion | Away | League | 2-2 | Dryden (2) | 13066 | |
| 26-Nov-49 | Southend United | Home | FAC r1 | 0-2 | | 17932 | |
| 3-Dec-49 | Millwall | Away | League | 1-3 | McGeachy | 16517 | |
| 17-Dec-49 | Watford | Away | League | 1-2 | Sutherland | 9500 | |
| 24-Dec-49 | Reading | Home | League | 2-1 | Sutherland (2) | 9099 | |
| 26-Dec-49 | Bristol Rovers | Home | League | 1-0 | Banner (pen) | 11761 | |
| 27-Dec-49 | Bristol Rovers | Away | League | 0-3 | | 19786 | |
| 31-Dec-49 | Torquay United | Away | League | 1-4 | Sutherland | 7575 | |
| 14-Jan-50 | Exeter City | Away | League | 1-1 | Clark (og) | 8917 | |
| 21-Jan-50 | Ipswich Town | Home | League | 4-0 | Sutherland (3), Blair | 9826 | |
| 28-Jan-50 | Walsall | Home | League | 2-2 | Sutherland, Pullen | 7251 | |
| 4-Feb-50 | Port Vale | Away | League | 0-2 | | 14176 | |
| 11-Feb-50 | Swindon Town | Home | League | 1-3 | Stroud | 9955 | |
| 18-Feb-50 | Notts County | Home | League | 1-4 | Sutherland | 21633 | |
| 25-Feb-50 | Aldershot | Home | League | 2-7 | Pullen, McEwan | 8797 | |
| 4-Mar-50 | Nottingham Forest | Away | League | 1-2 | McEwan | 19802 | |
| 11-Mar-50 | Northampton Town | Home | League | 1-0 | Sutherland | 9914 | |
| 18-Mar-50 | Norwich City | Away | League | 0-4 | | 20025 | |
| 25-Mar-50 | Newport County | Home | League | 2-1 | Campbell, Pattison | 8819 | |
| 1-Apr-50 | Bristol City | Away | League | 0-0 | | 16908 | |
| 7-Apr-50 | Bournemouth & Boscombe Athletic | Home | League | 2-1 | Pattison (2) | 13925 | |
| 8-Apr-50 | Brighton & Hove Albion | Home | League | 0-1 | | 10276 | |
| 10-Apr-50 | Bournemouth & Boscombe Athletic | Away | League | 1-4 | Pattison | 9832 | |
| 15-Apr-50 | Walsall | Away | League | 2-1 | Pullen, Blair | 7347 | |
| 22-Apr-50 | Millwall | Home | League | 1-1 | Sutherland | 16308 | |
| 29-Apr-50 | Swindon Town | Away | League | 1-0 | Blair | 5522 | |
| 6-May-50 | Southend United | Home | League | 2-2 | McEwan, Pullan | 13197 | |
